QC Pharma interview questions 20 Questions
1 . What is room temperature ?
ans : 25 Degree Centigrade
2 . What is the ultraviolte (uv) and visible spectroscopy ?
ans : UV spectroscopy range 200 -400 nm
Visible spectroscopy range 400 - 800 nm
3. Explain the principle of ultraviolate spectroscopy ?
ans : UV spectroscopy use light in the uv part of electromagnetic spectrum .
UV absorbtion spectra arise in which molecule or atoms outer electron absorb energy undergoes transition from lower energy level to higher energy level for each molecule absorbance at wavelength is specific .
4 . Expain about Lamberts Beer Law?
ans : it sates that the intentsity of monochromatic light absrobed by a substance dissoved in a fully transmitting solvent is driectly proprtional to the substance concentration and the path length of the light through the solution.
5 . What is body temperature ?
Ans : 37 Degree Centigrade or 98.6 F
6 . Define pH ? What is the pH of Blood ?
ans : pH : Negative logarithm of hydrogen ion concentartion
Blood pH : 7.35 to 7.45
7 . What is the HPLC principle ?
ans : it is a technique used for separating the mixture of compnent into individual components based on adsroption partition ion exchange and size exclusion principles .
Stationary phase and mobile phase used in it
HPLC used for identification quantification and purification of compounds from mixture .
8. In reversed phase HPLC which types of stationary phase is used and give example?
ans : Non polar stationary Phase used Silica gel C-18
9. What are the detectors used in HPLC ?
ans : UV VIS detectore , IR detector Fluorescence detector
Conductivity detector Mass Spectroscopy , LC MS Etc.
10 . How to calculate retention factor in paper chromatography .
Ans: Rf = Distance travelled by solute / Distance travelled by the solvent
11. Define molarity
Ans :Number of moles of solute per liter solution, denote with M
12 . Define molality .
Ans : Number of moles of solute per kilogram solvent .
denote with m
13. Define Normality ?
Ans ; Number of moles equivelant wight per liter solution.
14 . Difference between humidity and realative humidity ?
Ans : Humidity : measure of amount of water vapour present in the atmosphere,
Realative humidity : Water vapoure amount exists in air expresed as a % of the mount needed for saturation at the same temp.
15 . Expand LCMS ,HPLC , UPLC ,TLC and GC?
Ans. LCMS : Liquid chromatography
HPLC : High performance liquid chromatography
UPLC : Ultra high perfromance liquid chromatography .
TLC : Thin layer chromatography
GC : Gas chromatography
16 : Explain the infra red spectroscopy principle ?
Ans , When a molecule absorbs the infrared radiation it vibrates and gives rise to packed infrared .
Absorption spectrum this IR spectrum is specific for every different molecule absorbing the IR raditaon useful for its ientification.
17. What is the differance between qualitative and quantitative analysis?
Ans . Quatitative analysis involves identification of the compound or chemical based on their chemical or physical properties
e.g Melting point , Boiling point.
Quntitative analysise involves estimation or determination of concentration or amount of the chemical compound or components.
18 . What is the use of UV spectroscopy
Ans . Spectrosacopy used for detecting the
functional group impurities qualitative and quantitative analysis can be done .
19 . what do you mean by LOD and water content ?
Ans . Los on Drying (LOD) is determined by heating the sample below its melting point in an oven and it includes all volatile matter including water content and solvent .
% LOD = % water content % water mol
20. Explain the infrared spectroscopy principle ?
Ans . When a molecule absorbs the infrared radiation it vibrate and give rise to packed spctrum is specific for every different molecule absorbing the IR radiation .useful for its idifiaction
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